Understanding RPE Lipofuscin

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Understanding RPE lipofuscin.

In the Research Highlight article titled ‘‘Rethinking A2E,’’ Smith et al. offer interpretations of the recent paper by Ablonczy et al., titled ‘‘Lack of Correlation Between the Spatial Distribution of A2E and Lipofuscin Fluorescence in the Human Retinal Pigment Epithelium.’’ Readers will benefit from further discussion. Although not acknowledged by Smith et al., RPE lipofuscin consists of multi...

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Understanding RPE Lipofuscin.

In the recent paper by Allingham et al., fundus autofluorescence (AF) was studied in relation to progression of geographic atrophy (GA) in age-related macular degeneration (AMD), a subject of intense ongoing interest. The primary conclusion was that rim area focal hyperautofluorescence (RAFH) was positively correlated with GA progression rate (r 1⁄4 0.49, P < 0.001), consistent with Bearelly et...

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Photoreactivity of aged human RPE melanosomes: a comparison with lipofuscin.

PURPOSE To determine whether aging is accompanied by changes in aerobic photoreactivity of retinal pigment epithelial (RPE) melanosomes isolated from human donors of different ages, and to compare the photoreactivity of aged melanosomes with that of RPE lipofuscin. METHODS Human RPE pigment granules were isolated from RPE cells pooled into groups according to the age of the donors. Photoreact...

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Photooxidation of RPE lipofuscin bisretinoids enhances fluorescence intensity

Light-related cycling of chemically reactive vitamin A aldehyde leads to the formation autofluorescent bis-retinoid pigments that accumulate as lipofuscin in retinal pigment epithelial cells. The amassing of these diretinoid compounds is implicated in the pathogenesis of age-related macular degeneration and in some inherited forms of retinal degeneration. For all of these fluorophores, extended...

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Biosynthetic Studies of A2E, a Major Fluorophore of RPE Lipofuscin

∗ This work was supported by the National Institutes of Health Grants 34509 (KN) and EY12951 (JRS), the Charina Foundation (JRS), the Macula Vision Research Foundation (JRS) and unrestricted funds from Research to Prevent Blindness. JRS is a recipient of a Research to Prevent Blindness Lew R. Wasserman Merit Award. The costs of publication of this article were defrayed in part by the payment of...

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ژورنال

عنوان ژورنال: Investigative Opthalmology & Visual Science

سال: 2016

ISSN: 1552-5783

DOI: 10.1167/iovs.16-21081